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Then brain sections were incubated with rabbit monoclonal anti-HDAC9 antibody (Abcam, #ab109446, 1:200) in combination with one of following antibodies: mouse monoclonal anti-NeuN antibody (MilliporeSigma, #MAB377, 1:200), goat polyclonal anti-IBA1antibody (Abcam, #ab5076, 1:200) or mouse monoclonal anti-GFAP antibody (cell signaling technology, #3670, 1:200) diluted in TBST containing 10% donkey serum overnight at 4 C

Trigger Point Injections Needle insertion plus an injected solution (lidocaine, corticosteroid, or saline) Performed by physicians, nurse practitioners, and physician assistants Dual mechanism: mechanical disruption plus chemical effect Often provides faster initial relief due to the anesthetic component Better suited for patients with severe pain who need immediate relief Can include anti-inflammatory medication for inflammatory presentations Typically uses a larger gauge needle (22-25 gauge) Dry Needling Needle insertion without any injected solution Performed by physical therapists, chiropractors, and other trained providers Relies solely on mechanical disruption of the trigger point May require more needle manipulation to achieve full effect Preferred by patients who want to avoid any medication Often uses thinner acupuncture-style needles (filiform needles) Can be integrated into a physical therapy treatment session The bottom line: These are complementary approaches, not competing ones

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