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Moreover, the present invention provides for the modification of other properties, including its: Catalyzed reaction(s) Reaction type Natural substrate(s) Substrate spectrum Product spectrum Inhibitor(s) Cofactor(s)/prosthetic group(s) Metal compounds/salts that affect it Turnover number Specific activity Km value pH optimum pH range Temperature optimum Temperature range It is also instantly appreciated that the serviceability of a molecule with an immunogenic effect can be affected by additional physical properties, which can likewise be modified by directed evolution as provided herein, such as how it is affected by subjection to: Isolation/Preparation Purification Renaturation conditions (reversibility or retention of activity upon: heating and cooling, urea, salts, detergents, pH extremes) Crystallization pH Temperature Oxidation Organic solvent(s) Miscellaneous storage conditions Moreover, the instant invention provides for the modification of molecule's immunogenic properties such as Exposure to biological compartments (stomach acids, in vivo degradation) Expression (e.g

Much of this research originates from a specific scientific group, and independent replication is needed to confirm these effects

Acknowledgements This project was primarily funded by National Institutes of Health (NIH) grants R35GM156286 and R21NS127065 (to M.G-M)

however, because TPs are thermally destroyed, overly hot water extraction must be avoided

Recycling Other Antioxidants: This is the part most people don't know about

doi: 10.1016/j.mam.2012.11.006 215 SchangG.RobaireB.HalesB