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In 2011, plasma samples from the discovery cohort were collected from 60 unrelated individuals (31 unaffected, 29 konzo affected) in the Kahemba region of the Democratic Republic of the Congo, which is approximately 850 km southeast of the capital of Kinshasa

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This limitation poses a significant challenge to investigations of the neural mechanisms underlying ASD, given the distinct roles of Glu and Gln in the Gln-Glu cycle within the central nervous system