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In 1992, bisulfite sequencing emerged as a method to estimate methylation levels, and it quickly became the gold standard method for generating genome-scale methylation maps called methylomes [16, 23]

doi: 10.1152/physrev.00026.2013 23 FuchsKKuehnAMahlingMGuenthoerPHectorASchwenckJet al

Contraindications: Atropine generally is contraindicated in patients with , pyloric stenosis or prostatic hypertrophy, except in doses ordinarily used for preanesthetic medication

10.1016/j.cell.2013.12.010 151 YangW