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Presence of nitric oxide synthase activity in roots and nodules of Lupinus albus

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Ten grams of the fresh fungal biomass were minced in liquid N 2 , dispensed in 50 ml of 50 mM potassium phosphate buffer of pH 6.5 with 1 mM PMSF, and 1 mM EDTA, the homogenate was vigorously shacked for 5 min, centrifuged at 6000 rpm for 10 min, the supernatant was used as source of the crude enzyme [19, 23, 36]