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The spheres were washed once with PBS, then fixed in acetone, washed twice with PBS, and permeabilized using Triton X-100

Xie X, Yu H, Wang Y, Zhou Y, Li G, Ruan Z, Li F, Wang X, Liu H, Zhang J (2014) Nicotinamide N-methyltransferase enhances the capacity of tumorigenesis associated with the promotion of cell cycle progression in human colorectal cancer cells

Conversely, m 6 A density remains low in the 5UTR and the initial portion of the CDS, with a steady increase toward the terminal region

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