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[162] [242] They married in December 2012 in New York, and their son, Bear, was born the following year

Fixed cells were permeabilized and blocked for 1 h in goat serum dilution buffer (GSDB: 25% goat serum, 0.3% Triton X-100 in PBS)

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G-Biosciences IgG Binding, Wash & Elution Buffers are designed for the non-denaturing, high yield purification of antibodies from IgG affinity purification resins