3 Pieper, J.A
Through integrated proteomic and metabolomic analyses, we identified a high-inflammation, high-oxidation, low-metabolism triad in the hippocampus of CLP-induced SAE mice, providing novel insights into mechanisms underlying persistent cognitive deficits in SAE survivors
Other genes, including MW07R7 (a Glutathione S-transferase family protein) (Fig
Ferroptosis and tumor therapy Tumor cells require more iron than normal cells to support rapid proliferation
A method for the synthesis of a bifunctional complex comprising a molecule and a single stranded oligonucleotide identifier attached to the molecule, said method comprising the steps of i) providing a display oligonucleotide attached to a) one or more chemical reaction site(s) comprising one or more reactive groups and b) one or more priming site(s) for enzymatic addition of a tag, ii) providing a first reactant comprising one or more chemical entities and one or more reactive groups capable of reacting with c) the chemical reaction site(s) of the display oligonucleotide, and/or d) one or more reactive groups of at least a first further reactant comprising one or more chemical entities, wherein said first further reactant is provided simultaneously or sequentially in any order with the first reactant, iii) providing a first oligonucleotide tag capable of hybridising to part of a first oligonucleotide anti-tag, wherein the first oligonucleotide tag identifies the first reactant and, optionally, the further first reactant, iv) providing a first oligonucleotide anti-tag capable of hybridising to at least part of the first oligonucleotide tag provided in step iii) and to at least part of the display oligonucleotide provided in step i), v) reacting the first reactant provided in step ii) with c) the one or more chemical reaction site(s) of the display oligonucleotide and/or with d) the one or more reactive groups of the first further reactant comprising one or more chemical entities, wherein the reaction of complementary reactive groups result in the formation of a covalent bond, and wherein one or more reactive group reactions of step v) result in the formation of one or more covalent bond(s) between the chemical reaction site(s) of the display oligo and at least one chemical entity of at least one reactant selected from the group consisting of the first reactant and the further first reactant, vi) hybridising the anti-tag to the display oligonucleotide and to the first oligonucleotide tag, wherein method steps v) and vi) are simultaneous or sequential in any order, vii) enzymatically ligating the display oligonucleotide and the first oligonucleotide tag, viii) providing a second reactant comprising one or more chemical entities and one or more reactive groups capable of reacting with c) the chemical reaction site(s) of the display oligonucleotide, and/or d) one or more reactive groups of one or more reactant(s) having reacted in a previous synthesis round, and/or e) one or more reactive groups of at least a second further reactant comprising one or more chemical entities, wherein said second further reactant is provided simultaneously or sequentially in any order with the second reactant, ix) providing a second oligonucleotide tag capable of hybridising to part of a second oligonucleotide anti-tag, wherein the second oligonucleotide tag identifies the second reactant and, optionally, the further second reactant, x) providing a second oligonucleotide anti-tag capable of hybridising to part of the first oligonucleotide tag provided in step iii) and to part of the second oligonucleotide tag provided in step ix), xi) reacting the second reactant provided in step viii) with c) the chemical reaction site(s) of the display oligonucleotide and/or d) one or more reactive groups of one or more reactant(s) having reacted in a previous synthesis round and/or e) one or more reactive groups of a further second reactant comprising one or more chemical entities, wherein the reaction of complementary reactive groups result in the formation of a covalent bond, and wherein one or more reactive group reactions of step xi) result in f) the formation of one or more covalent bond(s) between the one or more chemical reaction site(s) and at least one chemical entity of at least one reactant selected from the group consisting of the second reactant and the further second reactant, and/or g) the formation of one or more covalent bond(s) between a reactant having reacted in a previous synthesis round and at least one chemical entity of at least one reactant selected from the group consisting of the second reactant and the further second reactant, xii) hybridising the anti-tag to the first oligonucleotide tag and the second oligonucleotide tag, wherein method steps xi) and xii) are simultaneous or sequential in any order, xiii) enzymatically ligating the first and second oligonucleotide tags in the absence of ligation the first and second anti-tag oligonucleotides, and optionally xiv) displacing unligated anti-tags from the bifunctional complex comprising a molecule and a single stranded oligonucleotide identifier comprising tags identifying the reactants which participated in the synthesis of the molecule
Effects vary from person to person
