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Benirschke K, Burton G, Baegen R (2012) Pathology of the human placenta

Interaction networks, GO, KEGG and INTERPRO domain analysis were done by STRING [78], the statistical environment R and Perseus [79]

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For fasting glucose and insulin measurements, serum was collected after 10 h of fasting (08:0018:00 h) by tail snip, glucose was measured using liquid chromatographymass spectrometry (LCMS) and insulin was measured using an Ultra Sensitive Mouse Insulin ELISA Kit (cat

At the endpoint, cells were washed three times with saline and stained for 20 minutes at room temperature using a saline solution containing 0.2 mM glutamic acid gamma-4-methoxy-beta-naphthylamide (GMNA, Sigma-Aldrich G0141), 20 mM glycyl-glycine (Sigma-Aldrich G1002), 25 mM Tris (Sigma-Aldrich 93362), and 1.2 mM FastBlue (Sigma-Aldrich F3378)